The actual causes of PCOS are still unclear, although high levels of male hormones in females can prevent the ovaries from producing hormones and making eggs properly. The levels of hormones in the blood can aid the diagnosis of PCOS and the tests that are carried out are Testosterone, TSH, Glucose, HbA1C, FSH, DHEAs, Insulin, LH, 17 Hydroxyprogesterone, Lipid Profile, Prolactin, Cortisol, Antimullerian Hormone, Androstenedione and SHBG tests.